smart seq library prep kit (TaKaRa)
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TaKaRa
smart seq library prep kit
Smart Seq Library Prep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smart+seq+library+prep+kit/Unique+Dual+Index+Kit+-+96U/us12629681-231-1-10
Average 94 stars, based on 16 article reviews
Smart Seq Library Prep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smart+seq+library+prep+kit/Unique+Dual+Index+Kit+-+96U/us12629681-231-1-10
Average 94 stars, based on 16 article reviews
smart seq library prep kit - by Bioz Stars,
2026-11
94/100 stars
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Sequencing:Article Title: Overexpression of placenta-specific noncanonical imprinted genes causes placental enlargement in intersubspecific hybrid mice Article Snippet: Total RNAs were purified from frozen embryos, reverse transcribed, and amplified using SMART-Seq v4 kits (Takara Bio). .. Sequencing libraries were prepared with the Article Title: Apparatuses and methods for performing multiple omics analysis and processing analyte mixtures Article Snippet: Following the transfer of samples into a 384-well plate containing RT-PCR buffer with 3′ SMART-Seq CDS Primer IIA (SMART-Seq® v4 PLUS Kit, TakaRa, cat #R400753); the samples were immediately denatured at 72° C. for 3 min and chilled on ice for at least 2 min. Full length cDNA was generated by adding RT mix to each tube and incubating at 42° C. for 90 min; followed by heat inactivation at 70° C. for 10 min. 18 cycles of cDNA amplification were done to generate enough cDNA for template library according to SMART-Seq® v4 PLUS Kit instruction. .. The Article Title: Overexpression of Placenta-Specific Noncanonical Imprinted Genes Causes Placental Enlargement in Intersubspecific Hybrid Mice. Article Snippet: Placental enlargement in somatic cell nuclear transfer (SCNT)-derived mice is attributed to biallelic expression of noncanonical (H3K27me3-dependent) imprinted genes owing to loss of imprinting (LOI).. Here, we investigated whether a similar mechanism underlies placental enlargement in intersubspecific hybrids between BDF1 (Mus musculus domesticus) and HMI (M. m. castaneus) mice.. Quantitative and allelic expression analyses revealed gene-specific LOI in (BDF1 × HMI)F1 placentas: Jade1 (Phf17) and Slc38a4 showed LOI in all placentas regardless of expression levels, whereas Gab1 and Sfmbt2 exhibited LOI only when expression levels were elevated. Generated:Article Title: The production of the chemokine CCL2 by corneal sensory neurons initiates anti-viral immunity at the cornea and trigeminal ganglion Article Snippet: Full-length cDNA was generated from single cells with the Takara SMART-Seq Single-cell kit (Takara: 634473) according to the manufacturer’s instructions. .. cDNA was assessed for quality using an Agilent Fragment Analyzer 5300 prior to libraries being generated by tagmentation with the Article Title: The production of the chemokine CCL2 by corneal sensory neurons initiates anti-viral immunity at the cornea and trigeminal ganglion. Article Snippet: Full-length single-cell RNA-seq library preparation and sequencing of corneal afferents Full-length cDNA was generated from single cells with the Takara SMART-Seq Single-cell kit (Takara: 634473) according to the manufacturer’s instructions. .. Briefly, tubes with cells were defrosted at 72 ◦ C for 3 min then held on ice prior to cDNA generation using 17 cycles for SMART whole genome amplification. cDNA was assessed for quality using an Agilent Fragment Analyzer 5300 prior to libraries being generated by tagmentation with the Amplification:Article Title: The production of the chemokine CCL2 by corneal sensory neurons initiates anti-viral immunity at the cornea and trigeminal ganglion Article Snippet: Full-length cDNA was generated from single cells with the Takara SMART-Seq Single-cell kit (Takara: 634473) according to the manufacturer’s instructions. .. cDNA was assessed for quality using an Agilent Fragment Analyzer 5300 prior to libraries being generated by tagmentation with the Article Title: The production of the chemokine CCL2 by corneal sensory neurons initiates anti-viral immunity at the cornea and trigeminal ganglion. Article Snippet: Full-length single-cell RNA-seq library preparation and sequencing of corneal afferents Full-length cDNA was generated from single cells with the Takara SMART-Seq Single-cell kit (Takara: 634473) according to the manufacturer’s instructions. .. Briefly, tubes with cells were defrosted at 72 ◦ C for 3 min then held on ice prior to cDNA generation using 17 cycles for SMART whole genome amplification. cDNA was assessed for quality using an Agilent Fragment Analyzer 5300 prior to libraries being generated by tagmentation with the other:Article Title: Enhancer Rewiring Orchestrates Inflammation and Loss of Cell Identity During Muscle Stem Cell Aging. Article Snippet: FISCs (~150,000 per sample) were seeded in each well of a chamber slide (Ibidi) that had been treated with 0.1 mg/mL polyD- lysine (Sigma) and 100 μg/mL of freshly prepared ECM gel (Sigma). Article Title: Enhancer Rewiring Orchestrates Inflammation and Loss of Cell Identity During Muscle Stem Cell Aging Article Snippet: After purification of amplified cDNA, quality and quantity were determined using a 2100 Bioanalyzer the DNA high sensitivity kit (HS, Agilent). Whole Genome Amplification:Article Title: The production of the chemokine CCL2 by corneal sensory neurons initiates anti-viral immunity at the cornea and trigeminal ganglion. Article Snippet: Full-length single-cell RNA-seq library preparation and sequencing of corneal afferents Full-length cDNA was generated from single cells with the Takara SMART-Seq Single-cell kit (Takara: 634473) according to the manufacturer’s instructions. .. Briefly, tubes with cells were defrosted at 72 ◦ C for 3 min then held on ice prior to cDNA generation using 17 cycles for SMART whole genome amplification. cDNA was assessed for quality using an Agilent Fragment Analyzer 5300 prior to libraries being generated by tagmentation with the Concentration Assay:Article Title: The role of TEAD4 in trophectoderm commitment and development is not conserved in non-rodent mammals Article Snippet: Once the genotype of each blastocyst was known, libraries were prepared for five WT and four KO samples, each corresponding to an individual blastocyst using SMART-Seq v4 PLUS kit (Takara Bio). .. Following cDNA quantification (Qubit, Thermo Fisher Scientific), samples were diluted to the same concentration and were processed using |